Evidence of cytotoxic activity against mammalian red blood cell of Na+ channel neurotoxin (Ae1) from sea anemone (Actinia equina)

Authors

  • M G Parisi Marine Immunobiology Laboratory, Department of Biological, Chemical and Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy
  • M R Trapani Marine Immunobiology Laboratory, Department of Biological, Chemical and Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy
  • L Cardinale Marine Immunobiology Laboratory, Department of Biological, Chemical and Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy
  • M Cammarata Marine Immunobiology Laboratory, Department of Biological, Chemical and Pharmaceutical Science and Technology, University of Palermo, Palermo, Italy

DOI:

https://doi.org/10.25431/1824-307X/isj.v13i1.309-314

Keywords:

Actinia equina, hemolysin, Na channel neurotoxin, sea anemone, Ae1 neurotoxin

Abstract

The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.

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Published

2016-09-19

Issue

Vol. 13 No. 1 (2016)

Section

Research Reports