Identification of BmBrat gene in silkworm, Bombyx mori

Authors

  • X X Ke State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • H Gao State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • K Chen Department 6 of Research Institute of Surgery, State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, Army Medical University, Chongqing 400042, P.R. China
  • L Zhang State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • S Chen State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • X Wang State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • Z Wei State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • L Yang State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China
  • H Cui State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China

DOI:

https://doi.org/10.25431/1824-307X/isj.v15i1.149-157

Keywords:

silkworm, BmBrat, gene expression profile, subcellular localization

Abstract

NHL protein family is evolutionarily conserved in either vertebrates or invertebrates. It has been extensively studied in mammals for the function of regulating cell proliferation and differentiation. Brat, known as the homologue of TRIM in insects, may have the same function. In this paper, we identified and characterized BmBrat in silkworm. The gene expression profile indicated that BmBrat was expressed in all tissues and the expression was especially high in ovary and head at larval stage. The expression profile was also detected at different stages of embryo development, and reached a peak at 4th and 5th days of the embryonic period. Subcellular localization of BmBrat in hemocytes revealed that it was specifically expressed in cytoplasm. Over-expression of BmBrat in silkworm significantly reduced the rate of cell proliferation by BrdU staining. This finding suggested that BmBrat played an important role in cell processes.

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Published

2018-04-07

Issue

Vol. 15 No. 1 (2018)

Section

Research Reports