Role of glutamate transporter EAAT2 in survival of silk worm Bombyx mori at higher dosages of Azaserine, an analogue of glutamine

Abstract

Organisms are subjected to a variety of stress and physiological impact in the event of drug
administration. Drug extrusion plays a critical role in the survival of organisms. The present study elucidate the role of glutamate transporter, EAAT2 in survival of model organism silkworm injected with azaserine, a glutamine analogue and offer a possible explanation for a contrasting observation of greater survival at higher dosage and lesser survival in lower dosage. Our results have shown a dosage dependent inhibition of gamma glutamyl transferase (GGT) activity, differential expression of EAAT2 transporter along with concomitant changes in residual concentration of azaserine, glutamate and cysteine that undermine cysteine transport. The current results also suggest that survival is dependent on EAAT2 transporter in cysteine-glutamate/azaserine transport and rejuvenation of ATP formation as observed in higher but not in lower dosage received worms.