The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri

Authors

  • M Wang CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  • L Wang Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, China
  • D Ni CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  • Q Yi Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, China
  • X Wang College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao 266042, China
  • Z Jia CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  • L Song Laboratory for Marine Fisheries Science and Food Production Processes, National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China ; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China ; Dalian Key Laboratory of Disease Prevention and Control for Aquaculture Animals, Dalian Ocean University, Dalian 116023, China

DOI:

https://doi.org/10.25431/1824-307X/isj.v15i1.302-315

Keywords:

Chlamys farreri, Glutathione S-transferase, innate immunity

Abstract

Glutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic
roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGSTω, CfGSTσ-1, CfGSTσ-2, CfGSTρ, CfGSTζ and CfmGST. CfGSTs shared high similarities with their counterparts from other species, and were clustered with their homologues into the corresponding clades in the phylogenetic tree, respectively. We investigated the distribution of their mRNA transcripts in different tissues and their temporal expression profiles in hemocytes after microbe stimulations by quantitative real-time PCR. The six CfGST genes were detectable in all the tested tissues, including hemocytes, muscle, mantle, gill, hepatopancreas, and gonad. Stimulations with various microbes drastically induced the mRNA transcripts of all the CfGSTs with different expression profiles. For examples, CfGSTω could be induced by three kinds of microbes, including Vibrio anguillarum, Micrococcus luteus and Pichia pastoris, whereas CfmGST could be only induced by V. anguillarum. These results indicated a powerful detoxification system of GSTs in scallop. Moreover, the distinct mRNA expression profiles of CfGSTs indicated their versatile and immune-challenge specific roles in the mollusk C. farreri.

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Published

2018-08-27

Issue

Vol. 15 No. 1 (2018)

Section

Research Reports