Comparative study of two novel extracellular copper/zinc superoxide dismutase (Cu-Zn SOD) genes from white shrimp Litopenaeus vannamei

Authors

  • J Sun MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China
  • Y Wang MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China ; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China
  • F Hu MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China
  • J J Hu MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China ; Laboratory for Marine Fisheries Science and Food Production Processes, and Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China
  • M Q Wang MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China ; Laboratory for Marine Fisheries Science and Food Production Processes, and Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China ; Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China

DOI:

https://doi.org/10.25431/1824-307X/isj.v19i1.126-135

Keywords:

Litopenaeus vannamei, extracellular Cu-Zn SOD, innate immunity

Abstract

Reactive oxygen species (ROS) is a product of normal metabolism of aerobic cells, but excess ROS is harmful to the organism. Copper/zinc superoxide dismutase (Cu-Zn SOD) is a metalloenzyme for scavenging ROS. In this study, two extracellular Cu-Zn SOD genes (designated as LvEcSOD1 and LvEcSOD2) were cloned by rapid amplification of cDNA ends (RACE) technique. The cDNA length of LvEcSOD1 is 801 bp, with an open reading frame (ORF) of 555 bp, encoding a peptide of 184 amino acids. The cDNA length of LvEcSOD2 is 934 bp with an ORF of 678 bp, encoding a peptide of 225 amino acids. The predicted amino acid sequences of the two LvEcSOD both contained conserved four Cu2+ binding sites and four Zn2+ binding sites. The mRNA scripts of LvEcSOD1 and LvEcSOD2 were widely detectable in the eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve, and stomach of Litopenaeus vannamei. Both LvEcSOD1 and LvEcSOD2 exhibited the highest expression levels in hemocytes and hepatopancreas. After the white shrimp was stimulated by white spot syndrome virus (WSSV) or Vibrio parahaemolyticus, the mRNA expression levels of these two genes were up-regulated to varying degrees. The relative expression level of LvEcSOD2 was significantly increased after stimulation by two pathogenic microorganisms, which was generally later than that of LvEcSOD1. These results indicated that the two genes are both involved in the innate immunity of L. vannamei with different functions.

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Published

2022-12-09

Issue

Vol 19 No 1 (2022)

Section

Research Reports