Sequence characterization and expression analysis of a PDGF/VEGF related factor gene in swimming crab Portunus trituberculatus

Authors

  • Y Gu MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China ; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute, Ocean University of China, Sanya 572024, China
  • Z Qu MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China ; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute, Ocean University of China, Sanya 572024, China
  • J-J Hu MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China ; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute, Ocean University of China, Sanya 572024, China ; Hainan Seed Industry Laboratory, Sanya 572024, China
  • M-Q Wang MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China ; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute, Ocean University of China, Sanya 572024, China ; Hainan Seed Industry Laboratory, Sanya 572024, China

DOI:

https://doi.org/10.25431/1824-307X/isj.v21i1.1-8

Keywords:

innate immunity, PDGF/VEGF related factor, Portunus trituberculatus, sequence features, expression profiles

Abstract

 

PDGF/VEGF factors are closely associated with immune defense systems. In this study, a PDGF/VEGF related factor gene (designated as PtPVF) from swimming crab Portunus trituberculatus was identified and analyzed. Its full-length cDNA is 1320 bp, containing an open reading frame (ORF) of 582 bp, 5’ untranslated regions (UTR) of 105 bp and 3’ UTR of 633 bp, which encoded a polypeptide of 193 amino acid residues. The deduced amino acid sequence of PtPVF contained a signal peptide, a typical PDGF/VEGF domain, and a cysteine knot motif (CXCXC). Quantitative real-time PCR analysis demonstrated that the mRNA of PtPVF was detectable in gills, gonads, heart, hemocytes, hepatopancreas, intestines, muscles, optic stalk, stomach, and the highest level was found in hemocytes, followed by gill and hepatopancreas. After stimulation by Vibrio alginolyticus, pentachlorophenol (PCP) or cadmium chloride (CdCl2), the relative mRNA expression levels of PtPVF in hemocytes reached their respective peaks at 3 h, 6 h and 6 h, and 36.61-fold, 3.06-fold and 21.26-fold compared with the original level, respectively, and the respond to V. alginolyticus and CdCl2 is earlier than to PCP, while under the challenge of V. alginolyticus and CdCl2, the mRNA expression level of PtPVF was significantly higher than that of PCP stimulation. Furthermore, the mRNA expression level of PtPVF first decreased at 3 h and then increased rapidly to a peak at 6 h after PCP challenge. All these results demonstrated that PtPVF might be involved in the response to invading pathogens and environmental pollution stimulants of P. trituberculatus.

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Published

2024-02-23

Issue

Vol. 21 No. 1 (2024)

Section

Research Reports